Method for preparing agent for prevention of hair loss and promotion of hair regrowth through fusion fermentation of lactic acid bacteria

A method for preparing an agent for prevention of hair loss and promotion of hair regrowth, wherein the method comprises a fusion fermentation of lactic acid bacteria comprising the steps of:

• (a) blending aerobic lactic acid bacteria raw materials by combining Lactobacillus sp. in an amount of 40 to 60 parts by weight with Saccharomyces cerevisae in an amount of 40 to 60 parts by weight, and diluting the combined raw materials with water such that the raw materials constitute 1 to 10 parts by weight of the total mixture;
• (b) adding molasses to the aerobic lactic acid bacteria raw materials by blending the raw materials with a molasses solution prepared by diluting 100 to 300 g of molasses in 10 L of water;
• (c) performing a first aerobic fermentation of the mixture obtained in step (b) at 15 to 35° C. for 10 to 15 days while introducing air into the mixture at 20 to 40 parts by weight per minute based on the total content of the mixture, thereby obtaining an aerobic lactic acid bacteria first culture solution;
• (d) maturing the aerobic lactic acid bacteria first culture solution in a sealed state at 15 to 35° C. for 10 to 15 days;
• (e) blending anaerobic lactic acid bacteria raw materials by combining Weissella sp. in an amount of 40 to 60 parts by weight with Bifidobacterium sp. in an amount of 40 to 60 parts by weight, and diluting the combined raw materials with water such that the raw materials constitute 1 to 10 parts by weight of the total mixture;
• (f) adding molasses to the anaerobic lactic acid bacteria raw materials by blending the raw materials with a molasses solution prepared by diluting 1 to 3 kg of molasses in 10 L of water;
• (g) performing a first anaerobic fermentation of the mixture obtained in step (f) at 15 to 35° C. for 10 to 15 days while introducing air into the mixture at 20 to 40 parts by weight per minute based on the total content of the mixture, thereby obtaining an anaerobic lactic acid bacteria first culture solution;
• (h) maturing the anaerobic lactic acid bacteria first culture solution in a sealed state at 15 to 35° C. for 10 to 15 days;
• (i) performing a second fermentation to prepare a mixed culture solution by mixing the matured aerobic lactic acid bacteria first culture solution with the matured anaerobic lactic acid bacteria first culture solution, blending the mixed culture solution with a molasses solution prepared by diluting 0.5 to 1.5 kg of molasses in 10 L of water, and fermenting the mixture at 25 to 45° C. for 10 to 15 days while introducing air into the mixture at 20 to 40 parts by weight per minute based on the total content of the mixture;
• and
• (j) maturing the fermented mixed culture solution in a sealed state at 25 to 45° C. for 10 to 15 days.