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US 12,590,273 B2Granted
Stabilized glycoside hydrolase variants
AssigneeNovozymes A/S
InventorsLars Giger, Frank Winther Rasmussen, Svend Gunnar Kaasgaard, Lars Anderson, Kenneth Jensen, Roland Alexander Pache, Dorte Marie Koefoed Klitgaard, Mette Louise Dissing Overgaard, Christian I. Joergensen
Life of this patent
Estimating the term…
Abstract
Disclosed are variants of a glycoside hydrolase having improved stability, e.g., in the presence of a protease, and the use of such variants in detergent applications, such as laundry or dish wash.
Claims27
A variant of a polypeptide having cellulase activity, wherein the variant comprises a catalytic domain, a linker region, and a carbohydrate binding module (CBM), wherein the variant has cellulase activity and wherein:
- a) the catalytic domain comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence as shown in positions 1-212 of SEQ ID NO:
- 1,
- b) the CBM comprises an amino acid sequence having at least 90% sequence identity to the amino acid sequence as shown in SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 173, SEQ ID NO:
- 174, SEQ ID NO:
- 175, SEQ ID NO:
- 176, SEQ ID NO:
- 177, SEQ ID NO:
- 178, SEQ ID NO:
- 179, SEQ ID NO:
- 180, SEQ ID NO:
- 181, SEQ ID NO:
- 182, SEQ ID NO:
- 183, SEQ ID NO:
- 184, SEQ ID NO:
- 185, SEQ ID NO:
- 186, SEQ ID NO:
- 187, SEQ ID NO:
- 188, SEQ ID NO:
- 189, SEQ ID NO:
- 190, SEQ ID NO:
- 191, SEQ ID NO:
- 192, SEQ ID NO:
- 193, SEQ ID NO:
- 194, SEQ ID NO:
- 195, SEQ ID NO:
- 196, SEQ ID NO:
- 197, SEQ ID NO:
- 198, SEQ ID NO:
- 199, or SEQ ID NO:
- 200;
- and
- c) the linker is selected from the group consisting of:
- (SEQ ID NO:
- 12)
- TTPPTPTPTPTPG
- (SEQ ID NO:
- 13)
- TTPTPPTPTPTPTPG
- (SEQ ID NO:
- 14)
- TTPTPTPPTPTPTPTPG
- (SEQ ID NO:
- 15)
- TTPTPTPTPPTPTPTPTPG
- (SEQ ID NO:
- 16)
- TPPTPPTPPTPPTPPTPPTPPTPPTPPTPPTPPG
- (SEQ ID NO:
- 17)
- TPTTPTTPTTPTG
- (SEQ ID NO:
- 18)
- TPTTPTTPTTPTTPTTPTG
- (SEQ ID NO:
- 19)
- SPSSPSSPSSPSG
- (SEQ ID NO:
- 20)
- SPSSPSSPSSPSSPSG
- (SEQ ID NO:
- 21)
- SPPSPPSPPSPPSPPG
- (SEQ ID NO:
- 22)
- SPPSPPSPPSPPSPPSPPSPPSPPSPPSPPG
- (SEQ ID NO:
- 23)
- PPSSPSSPSSPSSPSSPSSPSG
- (SEQ ID NO:
- 24)
- SPSPG
- (SEQ ID NO:
- 25)
- SPSPSPSPSPG
- (SEQ ID NO:
- 26)
- TPTPTPTPTPG
- (SEQ ID NO:
- 27)
- PPPP
- (SEQ ID NO:
- 28)
- PPPPP
- (SEQ ID NO:
- 29)
- PPPPPP
- (SEQ ID NO:
- 30)
- PPPPPPPG
- (SEQ ID NO:
- 31)
- PPPPPPP
- (SEQ ID NO:
- 32)
- PPPPPPPPG
- (SEQ ID NO:
- 33)
- PPPPPPPPPG
- (SEQ ID NO:
- 34)
- PPPPPPPPPPG
- (SEQ ID NO:
- 35)
- PPPPPPPPPPPG
- (SEQ ID NO:
- 36)
- PPPPPPPPPPPPPG
- (SEQ ID NO:
- 37)
- PEPTPEPTG
- (SEQ ID NO:
- 38)
- PEPTPEPTPEPTG
- (SEQ ID NO:
- 39)
- PEPTPEPTPEPTPEPTG
- (SEQ ID NO:
- 40)
- PEPTPEPTPEPTPEPTPEPTG
- (SEQ ID NO:
- 41)
- PSPTPSPTPSPTPSPTG
- (SEQ ID NO:
- 42)
- PSPTPSPTPSPTPSPTPSPTG
- (SEQ ID NO:
- 43)
- PQPTPQPTG
- (SEQ ID NO:
- 44)
- PDPTPDPTG
- (SEQ ID NO:
- 45)
- PRPTPEPTG
- (SEQ ID NO:
- 46)
- PQPTPEPTG
- (SEQ ID NO:
- 47)
- PSPNSPNSPNG
- (SEQ ID NO:
- 48)
- PEPTPRPTG
- (SEQ ID NO:
- 49)
- PQPTPEPTPQPTPEPTPQPTPEPTPQPTG
- (SEQ ID NO:
- 50)
- PDPTPDPTPDPTG
- (SEQ ID NO:
- 51)
- PQPTPQPTPQPTPQPTG
- (SEQ ID NO:
- 52)
- PQPTPEPTPQPTPEPTG
- (SEQ ID NO:
- 53)
- SPSPSPSPPPG
- (SEQ ID NO:
- 54)
- SPSPSPSPDPG
- (SEQ ID NO:
- 55)
- SPSPSPSPKPG
- (SEQ ID NO:
- 56)
- SPSPSPSPAPG
- (SEQ ID NO:
- 57)
- SPSPSPSPSPSG
- (SEQ ID NO:
- 58)
- SPSPSPSPSP
- (SEQ ID NO:
- 59)
- SPSPSPSPSPS
- (SEQ ID NO:
- 60)
- SPSPSPSPSPP
- (SEQ ID NO:
- 61)
- SPSPSPSPSPE
- (SEQ ID NO:
- 62)
- SPSPSPSPSPN
- (SEQ ID NO:
- 63)
- SPSPSPSPSPGG
- (SEQ ID NO:
- 64)
- SPSPSPSPSPK
- (SEQ ID NO:
- 65)
- PEPTPEPTP
- (SEQ ID NO:
- 66)
- PEPTPEPTR
- (SEQ ID NO:
- 67)
- PEPTPEPTPEPTP
- (SEQ ID NO:
- 68)
- PEPTPEPTPEPTPEPTPSPTG
- (SEQ ID NO:
- 69)
- PEPTPEPTPEPTPEPTPTPTG
- (SEQ ID NO:
- 70)
- PEPTPEPTPEPTPEPTPGPTG
- (SEQ ID NO:
- 71)
- PEPTPEPTPEPTPEPTPDPTG
- (SEQ ID NO:
- 72)
- PEPTPEPTPEPTPEPTPETG
- (SEQ ID NO:
- 73)
- PEPTPEPTPEPTPEPTPEPTD
- (SEQ ID NO:
- 74)
- PEPTPEPTE
- (SEQ ID NO:
- 75)
- PEPTPEPTPEPTPEPTPEP
- (SEQ ID NO:
- 76)
- PEPTPEPTPEPTPEPTPSPT
- (SEQ ID NO:
- 77)
- PEPTPEPTPEPTPEPTPRPTT
- (SEQ ID NO:
- 78)
- PEPTPEPTPEPTPEPTPEPTT
- (SEQ ID NO:
- 79)
- PEPTPEPTPEPTPEPTPEPT
- (SEQ ID NO:
- 80)
- PEPTPEPTPEPTPEPTPEPTS
- (SEQ ID NO:
- 81)
- PEPTPEPTPEPTPEPTPEPTR
- (SEQ ID NO:
- 82)
- PPPGGPGGPGTPTSTAPGSGPTSPGGGSG
- (SEQ ID NO:
- 83)
- PPPGGPGGTGTPTSTAPGSGPTSPGGGSG
- (SEQ ID NO:
- 84)
- PPSGGPGGPGTPTSTAPGSGPTSPGGGSG
- (SEQ ID NO:
- 85)
- PEPTPRPTPEPTPRPTG
- (SEQ ID NO:
- 86)
- PKPTPEPTPKPTPEPTG
- (SEQ ID NO:
- 87)
- PEPTPKPTPEPTPKPTG
- (SEQ ID NO:
- 88)
- PEPTPQPTPEPTPQPTG
- (SEQ ID NO:
- 89)
- PRPTPEPTPRPTG
- (SEQ ID NO:
- 90)
- PKPTPEPTPKPTG
- (SEQ ID NO:
- 91)
- PEPTPQPTG
- (SEQ ID NO:
- 92)
- PEPTPQPTPEPTG
- (SEQ ID NO:
- 93)
- TPPTPPG
- (SEQ ID NO:
- 94)
- SPSSPSG
- (SEQ ID NO:
- 95)
- SPSSPSSPSG
- (SEQ ID NO:
- 96)
- TPTTPTG;
- and
- (SEQ ID NO:
- 97)
- TPTTPTTPTG
- wherein the variant has improved stability in comparison with the polypeptide of SEQ ID NO:
- 1 in an aqueous composition comprising a protease.
295 more elements
Dependencies are inferred from the claim text.