Processes and systems for preparing cellular or viral membranes and nanoparticles
AssigneeArytha Biosciences, LLC
InventorsWeiwei Gao, Che-Ming Jack Hu
Life of this patent
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Abstract
The present invention relates to processes and systems for preparing nanoparticles, cellular or viral membranes and/or cellular or viral membrane coated nanoparticles using or comprising, inter alia, a multi-inlet vortexing reactor, tangential flow filtration (TFF) and/or a high shear fluid processor such as a microfluidizer (or a microfluidizer processor). The present invention also relates to the nanoparticles, cellular or viral membranes and/or cellular or viral membrane coated nanoparticles prepared by the present processes and systems, and the uses and/or applications of the nanoparticles, cellular or viral membranes and/or cellular or viral membrane coated nanoparticles.
Claims20
A process for preparing a cellular or viral membrane, said process comprises:
- 1) lysing a cell, a cellular vesicle or a virus to obtain a composition comprising a cellular or viral membrane and a non-membrane cellular or viral moiety;
- and
- 2) subjecting said composition to tangential flow filtration (TFF) using a TFF filter device to separate said cellular or viral membrane from said non-membrane cellular or viral moiety, and
- wherein said TFF is conducted:
- using a feeding rate ranging about 0.1 mL/minute to about 1,000 mL/minute;
- using a feeding pressure ranging from about 0.1 psi to about 100 psi;
- using a retentate pressure ranging from about 0.1 psi to about 100 psi;
- and
- within a time ranging from about 10 minutes to about 10 hours for one production batch;
- and
- wherein said TFF filter device comprises a filtration membrane having a pore size ranging from about 1 nm to about 650 nm;
- and
- wherein said TFF is used to remove from about 50% to about 99.9999% of said non-membrane cellular or viral moiety and/or an organic solvent.
Dependencies are inferred from the claim text.