Antigen-binding molecules that bind CD38 and/or CD28, and uses thereof

A composition comprising a first nucleic acid molecule and a second nucleic acid molecule, wherein:

• a) the first nucleic acid molecule comprises a polynucleotide sequence encoding an HCVR of an isolated antibody or antigen-binding fragment thereof that binds to human CD38, wherein the HCVR comprises an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 4, an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 6, and an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 8;
• and the second nucleic acid molecule comprises a polynucleotide sequence encoding an LCVR of the antibody or antigen-binding fragment thereof that binds to human CD38, wherein the LCVR comprises an LCDR1 comprising the amino acid sequence of SEQ ID NO:
• 20, an LCDR2 comprising the amino acid sequence of AAS, and an LCDR3 comprising the amino acid sequence of SEQ ID NO:
• 24;
• or
• b) the first nucleic acid molecule comprises a polynucleotide sequence encoding an HCVR of an isolated antibody or antigen-binding fragment thereof that binds to human CD38, wherein the HCVR comprises an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 34, an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 36, and an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 38;
• and the second nucleic acid molecule comprises a polynucleotide sequence encoding an LCVR of the antibody or antigen-binding fragment thereof that binds to human CD38, wherein the LCVR comprises an LCDR1 comprising the amino acid sequence of SEQ ID NO:
• 50, an LCDR2 comprising the amino acid sequence of GAS, and an LCDR3 comprising the amino acid sequence of SEQ ID NO:
• 54.

A set of expression vectors comprising a first expression vector, a second expression vector, and a third expression vector, wherein:

• the first expression vector comprises a polynucleotide sequence encoding a first HCVR of a bispecific antigen binding molecule comprising an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 4, an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 6, and an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 8;
• the second expression vector comprises a polynucleotide sequence encoding a second HCVR of the bispecific antigen binding molecule comprising an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 12, an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 14, and an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 16;
• and
• the third expression vector comprises a polynucleotide sequence encoding an LCVR of the bispecific antigen binding molecule comprising an LCDR1 comprising the amino acid sequence of SEQ ID NO:
• 20, an LCDR2 comprising the amino acid sequence of AAS, and an LCDR3 comprising the amino acid sequence of SEQ ID NO:
• 24.

A set of expression vectors comprising a first expression vector, a second expression vector, and a third expression vector, wherein:

• the first expression vector comprises a polynucleotide sequence encoding a first HCVR of a bispecific antigen binding molecule comprising an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 34, an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 36, and an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 38;
• the second expression vector comprises a polynucleotide sequence encoding a second HCVR of the bispecific antigen binding molecule comprising an HCDR1 comprising the amino acid sequence of SEQ ID NO:
• 42, an HCDR2 comprising the amino acid sequence of SEQ ID NO:
• 44, and an HCDR3 comprising the amino acid sequence of SEQ ID NO:
• 46;
• and
• the third expression vector comprises a polynucleotide sequence encoding an LCVR of the bispecific antigen binding molecule comprising an LCDR1 comprising the amino acid sequence of SEQ ID NO:
• 50, an LCDR2 comprising the amino acid sequence of GAS, and an LCDR3 comprising the amino acid sequence of SEQ ID NO:
• 54.