CD3-specific binding molecules
AssigneeImmunocore Limited
InventorsMartina Canestraro, Nele Dieckmann, Stephen Harper, Peter Benedict Kirk, Rachel Mulvaney, Ronan O'Dwyer, Ian Butler Robertson
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Abstract
The present invention relates to specific binding molecules which bind to CD3, particularly antibodies and fragments thereof, with improved properties.
Claims19
An antibody or antibody fragment thereof that specifically binds to CD3, comprising an immunoglobulin variable light chain (VL) domain and an immunoglobulin variable heavy chain (VH) domain, wherein the immunoglobin VL domain comprises the sequence:
- (SEQ ID NO:
- 16)
- AIQMTQSPSSLSASVGDRVTITCRASQDIRNYLNWYQQKPGKAPKLLIY
- YTSRLESGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQQGNTLPWTF
- GQGTKVEIK;
- and
- the immunoglobulin VH domain comprises the sequence:
- (SEQ ID NO:
- 18)
- EVQLVESGGGLVQPGGSLRLSCAASGYSFTGYAMNWVRQAPGKGLEWVAL
- INPYKGVSTYNQKFKDRFTISVDKSKNTAYLQMNSLRAEDTAVYYCARSG
- YYGDSDWYFDVWGQGTLVTVSS;
- or
- (SEQ ID NO:
- 19)
- EVQLVESGGGLVQPGGSLRLSCAASGYSFTGYAMNWVRQAPGKGLEWVAL
- INPYKGVSTYNQKFKDRFTFSVDKSKNTAYLQMNSLRAEDTAVYYCARSG
- YYGDSDWYFDVWGQGTLVTVSS.
An antibody or antigen binding fragment thereof that specifically binds to CD3 comprising an immunoglobulin VL domain comprising Complementarity Determining Regions (CDRs) VLCDR1, VLCDR2, and VLCDR3, and an immunoglobulin VH domain comprising CDRs VHCDR1, VHCDR2, and VHCDR3, wherein the amino acid sequence of;
- VLCDR1 is
- (SEQ ID NO:
- 1)
- QDIRNY;
- VLCDR2 is
- YTS;
- VLCDR3 is
- (SEQ ID NO:
- 2)
- QQGNTLPWT;
- VHCDR1 is
- (SEQ ID NO:
- 3)
- GYSFTGYA;
- VHCDR2 is
- (SEQ ID NO:
- 4)
- INPYKGVS;
- and
- VHCDR3 is
- (SEQ ID NO:
- 5)
- ARSGYYGDSDWYFDV.
A heterodimeric TCR-anti-CD3 antibody fusion molecule, comprising:
- (a) a first polypeptide chain that comprises a TCR alpha chain variable domain;
- and
- (b) a second polypeptide chain that comprises a TCR beta chain variable domain fused to an anti-CD3 scFv having the amino acid sequence:
- (SEQ ID NO:
- 20)
- AIQMTQSPSSLSASVGDRVTITCRASQDIRNYLNWYQQKPGKAPKLLIY
- YTSRLESGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQQGNTLPWTF
- GQGTKVEIKGGGGSGGGGSGGGGSGGGGSGGGSEVQLVESGGGLVQPGG
- SLRLSCAASGYSFTGYAMNWVRQAPGKGLEWVALINPYKGVSTYNQKFK
- DRFTISVDKSKNTAYLQMNSLRAEDTAVYYCARSGYYGDSDWYFDVWGQ
- GTLVTVSS;
- or
- (SEQ ID NO:
- 21)
- AIQMTQSPSSLSASVGDRVTITCRASQDIRNYLNWYQQKPGKAPKLLIY
- YTSRLESGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQQGNTLPWTF
- GQGTKVEIKGGGGSGGGGSGGGGSGGGGSGGGSEVQLVESGGGLVQPGG
- SLRLSCAASGYSFTGYAMNWVRQAPGKGLEWVALINPYKGVSTYNQKFK
- DRFTFSVDKSKNTAYLQMNSLRAEDTAVYYCARSGYYGDSDWYFDVWGQ
- GTLVTVSS.
Dependencies are inferred from the claim text.