MHC class II molecules and methods of use thereof
AssigneeUniversity Health Network
InventorsNaoto Hirano, Kenji Sugata
Life of this patent
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Abstract
The present disclosure is directed to HLA class II molecules having a higher affinity for CD4 than naturally occurring HLA class II molecules. In certain aspects, the HLA class II molecule comprises a DQ beta chain having (i) an amino acid other than leucine at a position corresponding to amino acid residue 114 of SEQ ID NO: 1, (ii) an amino acid other than valine at a position corresponding to amino acid residue 143 of SEQ ID NO: 1, (iii) or both (i) and (ii). Certain aspects of the present disclosure are directed to nucleic acid molecules encoding the HLA class II molecules, vectors comprising the nucleic acid molecule, cells comprising the same, and methods of use thereof.
Claims14
A DQ beta chain comprising an amino acid sequence with:
- at least 80% identity to the amino acid sequence of SEQ ID NO:
- 3 and
- (i) a tryptophan at the position corresponding to amino acid residue 114 of SEQ ID NO:
- 3;
- (ii) a methionine at the position corresponding to amino acid residue 143 of SEQ ID NO:
- 3;
- (iii) a glutamine at the position corresponding to amino acid residue 110 of SEQ ID NO:
- 3;
- (iv) a valine at the position corresponding to amino acid residue 116 of SEQ ID NO:
- 3;
- (v) a histidine at the position corresponding to amino acid residue 118 of SEQ ID NO:
- 3;
- and
- (vi) an asparagine at the position corresponding to amino acid residue 146 of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.