Peptide synthesis and system thereof
AssigneePeptisystems AB
InventorsRannar Sillard, Mats Israelsson, Ulf Tedebark, Lars Holmberg
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Abstract
Processes and systems related to solid phase peptide synthesis are described. The processes include a flow-through process comprising cyclic addition of amino acids to a column packed with resin, wherein each cycle includes the combination of the amino acids with one or more reagents to provide an activated amino acid mixture, and wherein heating is applied to the amino acid(s) before they are passed through the column and wherein the amino acids are re-circulated at least once over the column packed with resin. Systems for such processes are also described.
Claims18
A flow-through process for solid phase peptide synthesis (SPPS) including cyclic addition of at least one amino acid to a column packed with resin, wherein each cycle includes the combination of amino acid(s) with one or more reagents to provide an amino acid mixture which is passed through the column, which method comprises:
- heating the amino acid mixture before being passed through the column;
- passing the amino acid mixture through the column packed with resin;
- and
- recirculating the amino acid mixture at least once over the column packed with resin;
- wherein, in each cycle, peptides are synthesized by linking at least one amino acid from the mixture to at least one amino acid immobilized to the resin;
- and
- wherein the heated amino acid mixture is passed through the column at a flow rate and in a volume that allow for their contact with at least 99% of immobilized and growing peptides.
Dependencies are inferred from the claim text.