US 12,589,391 B2 · Sample holders, PCR station assemblies, and methods of operating PCR testing system

Abstract

A sample holder for PCR processing. The sample holder includes a body with an inlet and outlet grooves formed alongside each other, a detection recess that is connected to the inlet and outlet grooves, and a fill port interconnected to both the inlet and outlet grooves, and a cover interfacing with the body to form an inlet channel interconnected to the fill port, a detection region interconnected to the inlet channel, and an outlet channel interconnected to the detection region and the fill port. The detection region is configured to receive a PCR solution from the fill port and replication occurs within the detection region via heating and cooling cycles. Thereafter, fluorescent emissions from tagged replicated DNA/RNA in the detection region are detected and measured. PCR stations, PCR station assemblies, PCR testing systems, and methods of operating a PCR testing systems are provided, as are other aspects.

Claims15

Dependent claims

A sample holder, comprising:

a body having a top surface and a bottom surface, the body further comprising:
an inlet groove formed into the bottom surface;
an outlet groove formed into the bottom surface alongside the inlet groove;
a detection recess formed into the bottom surface and connected to the inlet groove and the outlet groove;
a fill port interconnected to both the inlet groove and the outlet groove, the fill port having a top surface that extends above the top surface of the body and comprising an inlet port connected to the inlet groove and an outlet port connected to the outlet groove;
and
a cover connected to the bottom surface wherein the cover interfaces with the body to form an inlet channel interconnected to and positioned between the inlet port of the fill port and a detection region, and an outlet channel interconnected to and positioned between the detection region and the outlet port of the fill port.

4 more elements

Dependencies are inferred from the claim text.

Sample holders, PCR station assemblies, and methods of operating PCR testing system

Life of this patent

Sample holders, PCR station assemblies, and methods of operating PCR testing system

Life of this patent

Abstract

Claims15