Immunity-inducing agent comprising antigen peptide-adjuvant nucleotide conjugate and pharmaceutical composition comprising same
AssigneesThe University of Kitakyushu, Daiichi Sankyo Company, Limited
InventorsShinichi Mochizuki, Makoto Koizumi, Koji Morita
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Abstract
The present invention provides an immunity-inducing agent comprising, as an active component, a polynucleotide/peptide conjugate in which a single-chain polynucleotide or polynucleotide derivative comprising a CpG motif, and an antigenic peptide are bound via a spacer, wherein the spacer is covalently bound at one end thereof to the polynucleotide or polynucleotide derivative and covalently bound at the other end thereof to the antigenic peptide, as well as a pharmaceutical composition comprising said immunity-inducing agent.
Claims27
An immunity-inducing agent comprising, as an active component, a conjugate comprising (i) a single-chain polynucleotide or a single-chain polynucleotide derivative comprising a CpG motif, and (ii) an antigenic peptide bound thereto via a spacer, wherein the spacer is covalently bound at one end thereof to the single-chain polynucleotide or the single-chain polynucleotide derivative comprising a CpG motif and covalently bound at the other end thereof to the antigenic peptide,
- wherein the single-chain polynucleotide or the single-chain polynucleotide derivative comprising a CpG motif is not complexed with β-1,3-glucan,
- wherein the antigenic peptide and the spacer are bound together via a disulfide bond produced by a reaction between a thiol group of a cysteine residue at the N-terminus of the antigenic peptide and a thiol group of the spacer, and
- wherein the spacer comprises a repeating unit represented by the formula:
- wherein
- X represents an oxygen atom or a sulfur atom, wherein each X may be the same or different,
- R represents any of (CH2)pO, (CH2)qNH, and (CH2CH2O)m, wherein m, p and q each independently represent a natural number of not more than 10, and
- n represents a natural number of not more than 10.
A pharmaceutical composition comprising a conjugate comprising (i) a single-chain polynucleotide or a single-chain polynucleotide derivative comprising a CpG motif, and (ii) an antigenic peptide bound thereto via a spacer, wherein the spacer is covalently bound at one end thereof to the single-chain polynucleotide or the single-chain polynucleotide derivative comprising a CpG motif and covalently bound at the other end thereof to the antigenic peptide,
- wherein the single-chain polynucleotide or the single-chain polynucleotide derivative comprising a CpG motif is not complexed with β-1,3-glucan,
- wherein the antigenic peptide and the spacer are bound together via a disulfide bond produced by a reaction between a thiol group of a cysteine residue at the N-terminus of the antigenic peptide and a thiol group of the spacer, and
- wherein the spacer comprises a repeating unit represented by the formula:
- wherein
- X represents an oxygen atom or a sulfur atom, wherein each X may be the same or different,
- R represents any of (CH2)pO, (CH2)qNH, and (CH2CH2O)m, wherein m, p and q each independently represent a natural number of not more than 10, and
- n represents a natural number of not more than 10.
Dependencies are inferred from the claim text.