Systems to produce B cells genetically modified to express selected antibodies
AssigneeFred Hutchinson Cancer Center
InventorsJustin J. Taylor, Howell F. Moffett
Life of this patent
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Abstract
Systems and methods to genetically modify B cells to express selected antibodies are described. The systems and methods can be used to: obviate the need for classical vaccinations; provide protection against infectious agents for which no vaccinations are currently available; provide protection against infectious agents when patients are otherwise immune-suppressed; and/or provide a benefit provided by a therapeutic antibody, such as in the treatment of autoimmune disorders.
Claims20
A genetic construct comprising or encoding (i) a heavy chain promoter, (ii) a signal peptide, (iii) a full-length light chain of a selected antibody;
- (iv) a flexible linker or a skipping element;
- (v) a variable region of a heavy chain of the selected antibody;
- and (vi) a splice junction comprising the sequence CAGGTAAGT or CAGGTGAGT and 40-80 base pairs of the intron following the last exon of a variable-diversity-joining (VDJ).
Dependencies are inferred from the claim text.