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US 12,589,140 B2Granted
Antigen-presenting neutrophil-derived dendritic cells and methods of use thereof
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsTanya Mayadas, Vijaya Mysore, Xavier Cullere, Jon C. Aster
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Abstract
Methods and compositions for use in generating or promoting an immune response to cancer or an infection, comprising promoting differentiation of neutrophils into dendritic cells using a combination of GMCSF and (i) an immune complex comprising an antigen and an antibody comprising an Fc region that binds to FcγRIIA or FcγRIIIB, (ii) a conjugate comprising an antigen and an anti-FcγRIIIB antibody, or (iii) an anti-FcγRIIIB antibody.
Claims14
A method of generating a population of neutrophil-derived dendritic cells (NDDCs), the method consisting of:
- providing a population of neutrophils comprising neutrophils that express one or both of FcγRIIA and/or FcγRIIIB;
- contacting the neutrophils in culture with Granulocyte-macrophage colony-stimulating factor (GM-CSF) and an immune complex (IC) comprising an antigen and an antibody, wherein the antibody comprises an antigen binding portion that binds to the antigen and an Fc region that binds to FcγRIIA and/or FcγRIIIB;
- and
- maintaining the population of neutrophils in culture in the presence of GM-CSF together with IC for at least two days such that the neutrophils differentiate into NDDCs.
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Dependencies are inferred from the claim text.