Method for obtaining occlusive biological dressing, occlusive biological dressing, use thereof and kit

A method for obtaining a temporary occlusive biological dressing, characterized by comprising a following steps:

• i) cleaning and trimming a tilapia skin, wherein the species of tilapia is Oreochromis niloticus;
• ii) successive steps of incubation of the skin with biocompatible detergent or incubation with bactericidal agent or both incubation with a bactericidal agent and a biocompatible detergent followed by rinsing and/or incubation with detoxifying solution and rinsing with physiological saline solution;
• and
• iii) freeze-drying the tilapia skin at a temperature in the range of −30° C. to −80° C., with internal pressure between 4.00 and 4.66 Pa and for the time from 2 to 24 h;
• wherein the biocompatible detergent is 3-[(3-cholamidopropyl)dimethylammonium]-1-propanesulfonate, or Polysorbate 20, or t-octylphenoxypolyethoxyethanol, or sodium deoxycholate or 4-nonylphenyl-polyethyleneglycol or a combination of these,
• wherein the bactericidal agent is chlorhexidine digluconate, or sodium chlorite, or cetylpyridinium chloride, or chloramine T and/or sodium dichloroisocyanurate or a combination of these;
• and
• wherein the detoxifying solution is acetic acid/acetate buffer, or glycine/HCl buffer, or citric acid/citrate buffer, or sodium monobasic phosphate/sodium dibasic phosphate buffer or a combination of these.