Gene delivery system and application thereof in preparation of drugs for treatment of tumors

A gene delivery system, comprising a nucleic acid vector and a delivery vehicle, wherein the nucleic acid vector is a self-replicating RNA encoding HNF4 alpha;

• the gene delivery system delivers the HNF4 alpha or the self-replicating RNA into tumor cells, induces differentiation of the tumor cells into normal mature cells, inhibits proliferation of the tumor cells, and/or induces apoptosis of the tumor cells;
• the self-replicating RNA has a 35-100 nt polyadenine tail and is an engineered alphavirus genome that includes a 5′-cap, a non-coding or untranslated region (5′ UTR), four genes encoding nonstructural proteins that enable RNA replication, a 26S subgenome promoter, a gene encoding the HNF4 alpha replacing structural protein sequences of the engineered alphavirus genome, and a 3′ non-coding or untranslated region (3′ UTR);
• and the delivery vehicle comprises lipid-based nanoparticles.