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US 12,589,073 B2Granted
Leveraging lipid-protein interactions to engineer spatial organization in cell-free systems
AssigneeNorthwestern University
InventorsJustin Alexander Peruzzi, Neha Prashant Kamat, Jan Steinkuehler
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Abstract
The present disclosure provides methods and compositions for controlling the localization of proteins in lipid structures. The disclosure also provides methods for selecting appropriate proteins for desired localization and distribution in lipid structures based on hydrophobic thickness.
Claims14
A method comprising:
- incubating together
- a) a plasmid encoding a first protein, the first protein having a first hydrophobic thickness;
- b) a cell-free protein synthesis system;
- and
- c) a lipid structure comprising a lipid bilayer, the lipid bilayer comprising a first domain having a second hydrophobic thickness;
- wherein the incubation is done under conditions that promote integration of the first protein into the first domain of the lipid bilayer,
- wherein the first protein traverses the lipid bilayer,
- wherein the first hydrophobic thickness and the second hydrophobic thickness have a difference of no greater than about 5 angstroms.
5 more elements
Dependencies are inferred from the claim text.