Three-dimensional printed bone defect repair scaffold and preparation method thereof

A preparation method of a 3D printed bone defect repair scaffold, comprising:

• step 1, dissolving a gelatin, sodium alginate, and a 58S bioglass in water to obtain a solution, wherein mass-to-volume concentrations of components in the solution are that:
• the gelatin is 16%, the sodium alginate is 6.5%, and the 58S bioglass is 8.5%;
• the 58S bioglass is in powder form and composed of silicon dioxide (SiO2), calcium oxide (CaO) and phosphorous pentoxide (P2O5) in mass percents of 58%, 33% and 9%, respectively;
• step 2, stirring the solution to obtain 3D printing slurry and then performing 3D printing, wherein the 3D printing was performed with a nozzle with an opening diameter of 0.41 millimeter (mm) at a printing speed of 6 millimeter per second (mm/s) under a pressure of 0.38 megapascals (Mpa) and a temperature of 29 degrees Celsius (C);
• and
• step 3, obtaining a semi-finished scaffold after the 3D printing, performing chemical crosslinking of the semi-finished scaffold with a calcium chloride solution for 8 minutes (min) to 15 minutes and then immersing in a glutaraldehyde solution for chemical crosslinking for 1.5 hours to 2.5 hours, and subsequently cleaning and lyophilizing, to thereby obtain the 3D printed bone defect repair scaffold;
• wherein the 3D printed bone defect repair scaffold is a multilayer cross-linked porous network structure, in which a first layer comprises a plurality of lines mutually parallel, a second layer comprises a plurality of lines mutually parallel and perpendicularly connected to an upper surface of the lines of the first layer, and a third layer comprises a plurality of lines mutually parallel and perpendicularly connected to an upper surface of the lines of the second layer;
• a diameter of each of the plurality of lines of each layer of the 3D printed bone defect repair scaffold is in a range of 300 micrometers (μm) to 500 μm, and a distance between adjacent ones of the plurality of lines of each layer of the 3D printed bone defect repair scaffold is in a range of 500 μm to 700 μm,
• wherein a contour of the 3D printed bone defect repair scaffold is one selected from the group consisting of a cylinder, a cuboid, and a cone;
• and an internal pore structure of the 3D printed bone defect repair scaffold in shape is one selected from the group consisting of a circle, a square, a trapezoid, a triangle, and a rhombus,
• wherein the 3D printed bone defect repair scaffold is sized and configured to promote formation of bone tissue.