Closed-loop elution system to evaluate patients with suspected or existing peripheral arterial disease

A closed loop generation and/or infusion system comprising a radionuclide generator, an activity detector, a controller, a dose calibrator, a pump, eluant source and an automated elution system controlled by software and hardware for generation and infusion of a radionuclide for administering a dose of generated radionuclide into a subject for diagnosing a peripheral arterial disease in a subject suffering from or suspected of suffering from peripheral arterial disease and/or metabolic disease by performing quantitative assessment of blood flow in lower extremities or limbs of the subject;

• wherein the quantitative assessment is performed by analyzing one or more images and providing a severity score;
• wherein the analysis of the one or more images is performed by using an automated system;
• wherein the closed loop generation and/or infusion system further comprises:
• a) calculating a dose of Rb-82 based on subject parameters, imaging system parameters, generation and/or infusion system parameters;
• b) administering the calculated dose of Rb-82 in a subject at rest and stress condition;
• c) image capturing by PET scanner;
• d) performing quantitative assessment of blood flow in lower extremities or limbs of the subject;
• e) analyzing one or more images and providing a severity score based on the assessment and presented in a numerical or percentage value of occluded or affected tissue;
• f) performing diagnosis or identify the subjects at risk of developing peripheral arterial disease;
• and
• g) generating the report,
• wherein the dose of radionuclide ranges from 0.01 milli-Becquerel to 10,000 milli-Becquerel.