Systems, devices, and methods for visualization of tissue and collection and analysis of data regarding same

A method of guiding debridement of a wound with an imaging system, the method comprising:

• positioning an excitation light source of an imaging system relative to a wound;
• illuminating the wound with excitation light emitted by the excitation light source to cause the wound to emit fluorescence;
• positioning an emissions filter of the imaging system over a portion of a mobile communication device;
• aligning the emissions filter with an image sensor of a camera of the mobile communication device;
• positioning the mobile communication device to align a field of view of the camera with the illuminated wound to detect the fluorescence emitted by the illuminated wound with the image sensor of the mobile communication device;
• viewing the fluorescence emitted by the illuminated wound in real time, wherein the fluorescence is indicative of a presence of bacteria in the wound;
• and
• while viewing the fluorescence emitted by the wound in real time, debriding the wound to remove bacteria from the wound.