Apparatus and method to convert a regular bright-field microscope into a PS-QPI system
OwnerAna Doblas, Jorge Garcia-Sucerquia +1inventors
InventorsAna Doblas, Jorge Garcia-Sucerquia, Rohan Isaac
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Abstract
An apparatus, system, and related methods for the conversion of a regular bright-field microscope into a PS-QPI (Polarization-Sensitivity and Quantitative Phase Imaging) microscope system. A regular bright-field microscope is converted by insertion of a polarizing element in the illumination path and a Fresnel biprism in the image space of the system. The converted system is suitable for real-time quantitative PS phase imaging in a broad number of biological applications aimed at understanding cell growth and dynamic changes occurring during physiological processes, as well as identification of cell/tissue screening and diagnosis.
Claims15
A method of measuring the polarimetric properties of birefringent samples, comprising the steps of:
- providing a polarization-sensitive quantitative phase imaging (PS-QPI) system comprising
- a microscope comprising an illumination source, a microscope objective lens and a tube lens, configured to magnify an image of a sample;
- a polarizing element configured to orient a polarization state of the illumination source;
- a Fresnel biprism;
- and
- a sensor or detector;
- sequentially rotating the polarizing element in a controlled way to change the illumination polarization state;
- generating and recording holograms for each orientation angle of the polarizing element;
- reconstructing an unwrapped phase map for each orientation angle;
- selecting maximum and minimum values of the reconstructed phase map to generate an ordinary phase map and an extraordinary phase map for each orientation angle;
- and
- calculating phase difference between the extraordinary phase map and the ordinary phase map to generate a retardance map.
Dependencies are inferred from the claim text.