Composition and methods for assessing sensitivity and specificity of antibody detection reagents

A method to determine the specificity and sensitivity of an anti-human globulin antibody, the method comprising:

• a) binding the anti-human globulin antibody to a panel of human antibodies, wherein the human antibodies within the panel are of different Ig isoallotypes of IgG1, IgG2, IgG3, or IgG4 and comprise:
• a heavy chain comprising:
• complementarity determining region (CDR) 1 of SEQ ID NO:
• 120,
• CDR2 of SEQ ID NO:
• 121, and
• CDR3 of SEQ ID NO:
• 122;
• and
• a light chain comprising:
• CDR1 of SEQ ID NO:
• 123,
• CDR2 of SEQ ID NO:
• 124, and
• CDR3 of QQS, and
• b) detecting the binding of the anti-human globulin antibody to the human antibodies of the panel, thus determining the specificity and sensitivity of the anti-human globulin antibody to each of the different Ig isoallotypes of IgG1, IgG2, IgG3, or IgG4.