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US 12,584,928 B2Granted
Methods for detecting estradiol by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsMildred M. Goldman, Nigel J. Clarke, Richard E. Reitz
Life of this patent
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Abstract
Provided are methods for determining the amount of estradiol in a sample using mass spectrometry. The methods generally involve ionizing estradiol in a sample and detecting and quantifying the amount of the ion to determine the amount of estradiol in the sample.
Claims4
A method for determining the amount of estradiol in a plasma or serum sample, the method comprising:
- (a) adding ammonium sulfate to the plasma or serum sample prior to purification with liquid chromatography, wherein the ammonium sulfate is added in an amount sufficient to free estradiol from a protein that is present in the plasma or serum sample without precipitating the protein;
- (b) purifying estradiol from the plasma or serum sample by liquid chromatography to form a purified estradiol;
- (c) ionizing the purified estradiol to produce one or more ions detectable by tandem mass spectrometry;
- and
- (d) detecting the amount of the one or more ions by tandem mass spectrometry, wherein said one or more ions comprise an ion with a mass/charge ratio of 183.10±0.5 or 145.10±0.5;
- wherein the amount of the one or more ions is related to the amount of estradiol in the plasma or serum sample.
3 more elements
Dependencies are inferred from the claim text.