Multiplex detection in high resolution devices through measurement of localized fluorescence ratios

A system for detecting one or more target analytes in a sample the system comprising:

• a sample plate configured to hold the sample on a sample plate surface, the sample plate comprising a plurality of capture structures attached to the sample plate surface, each of the plurality of capture structures independently comprising:
• a detection portion configured to couple with a target analyte of the one or more target analytes;
• and
• a stem comprising a capture structure code, the capture structure code comprising a plurality of flowers, each independently comprising a piece of nucleic acid or polymer coupled to at least one detectable molecule, wherein the detectable molecules embody the capture structure code, and wherein the detectable molecules in combination uniquely identify a type of the capture structure associated with the detection portion;
• an excitation source configured to generate excitation energy delivered into the sample plate;
• a detector configured to detect light emitted by the detectable molecules;
• and
• a hardware processor operably connected to the detector and configured, when executing instructions stored in a memory, to:
• determine a location of each of the capture structures on the sample plate surface;
• read the capture structure code of each of the capture structures;
• determine a location at which a target analyte is bound to one of the capture structures;
• and
• determine the target analyte based on the capture structure code of the capture structure at the location at which the target analyte is bound to one of the capture structures.