Method and device for determining biological analytes
AssigneePaia Biotech GmbH
InventorSebastian Giehring
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Abstract
The invention relates to a method for quantitatively determining biological analytes in an aqueous solution in the presence of one or more functionalised surfaces, wherein the aqueous solution comprises at least one type of biological analyte and at least one type of fluorescene marker, characterised in that the quantity and/or concentration of the biological analyte or analytes is determined by measuring the florescence emission of the unbound fluorescence markers, as well as to a device for carrying out said method.
Claims13
A measuring chamber for quantitatively determining biological analytes in an aqueous solution in the presence of one or more functionalised surfaces, comprising a device and at least one side wall surrounding the device,
- wherein a base of the device can form a base of the measuring chamber and wherein the device has an at least partly transparent structural element, and
- wherein the base of the device, apart from a base area of the structural element, is opaque.
A microplate for quantitatively determining biological analytes in an aqueous solution in the presence of one or more functionalised surfaces,
- wherein the microplate comprises at least one measuring chamber comprising a device and at least one side wall surrounding the device,
- wherein a base of the device can form a base of the measuring chamber,
- wherein the device has an at least partly transparent structural element, and
- wherein the base of the device, apart from a base area of the structural element, is opaque.
A microplate for quantitatively determining biological analytes in an aqueous solution in the presence of one or more functionalised surfaces,
- wherein the microplate comprises a plurality of wells, each well represents a measuring chamber,
- wherein there is introduced into at least one well of the microplate a device which has an at least partly transparent structural element which is in a form of an upwardly tapering protrusion and a cross section of which can have any desired geometry,
- wherein an end of the structural element that is remote from a base of the device is such that substantially no test components settle there, and
- wherein edges of the well form the side walls of a respective measuring chamber,
- wherein the base of the device, apart from a base area of the structural element, is opaque.
Dependencies are inferred from the claim text.