Immunoassay method and immunoassay apparatus
AssigneeDenka Company Limited
InventorMiwa Kuwahara
Life of this patent
Estimating the term…
Abstract
Provided are a highly-sensitive antibody and a test reagent using the antibody. A method for detecting an analyte according to an immunoassay method, using a carrier on which an antigen, an antibody or an antigen-binding fragment thereof is immobilized, wherein the analyte is detected with increasing detection sensitivity by using a carrier to which an antigen, an antibody or an antigen-binding fragment thereof was immobilized by adding disaccharide and sugar alcohol to a solution comprising an antigen, an antibody or an antigen-binding fragment thereof.
Claims5
A method for detecting RS virus N protein in a specimen sample by immunochromatography, comprising
- contacting the specimen sample with an anti-RS virus N protein antibody or an antigen-binding fragment thereof,
- wherein the anti-RS virus N protein antibody or an antigen-binding fragment thereof is immobilized to a carrier by adding trehalose and mannitol to a solution comprising the anti-RS virus N protein antibody or antigen-binding fragment thereof, and
- wherein detection sensitivity is increased relative to that achieved in the absence of trehalose and mannitol.
An RS virus N protein detection reagent comprising an immobilized anti-RS virus N protein antibody or an antigen-binding fragment thereof,
- wherein the detection reagent is an immunochromatography test piece,
- wherein trehalose and mannitol are comprised in a detection region comprising the anti-RS virus N protein antibody or antigen-binding fragment thereof, and
- wherein detection sensitivity of the detection reagent is increased relative to that achieved in the absence of trehalose and mannitol.
A method for producing an RS virus N protein detection reagent, comprising
- contacting a solution comprising an anti-RS virus N protein antibody or antigen-binding fragment thereof with trehalose and mannitol, thereby immobilizing the anti-RS virus N protein antibody or antigen-binding fragment thereof,
- wherein the detection reagent is an immunochromatography test piece, and
- wherein the detection reagent has increased detection sensitivity relative to that achieved in the absence of trehalose and mannitol.
Dependencies are inferred from the claim text.