Method, kit, and sensor for detecting antibody of interest in wastewater
AssigneeTohoku University
InventorDaisuke Sano
Life of this patent
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Abstract
A method of detecting an antibody of interest in wastewater includes: bringing an electrode, modified with a protein that specifically binds with the antibody of interest, into contact with the wastewater; bringing the electrode into contact with a reaction solution containing a redox substance; and applying a voltage to the electrode in a state of being in contact with the reaction solution, and measuring a current.
Claims10
A method of detecting an antibody of interest as an infectious disease marker in wastewater, the method comprising:
- bringing an electrode, modified with a protein that specifically binds with the antibody of interest as the infectious disease marker, into contact with the wastewater;
- lifting the electrode out of the wastewater;
- bringing the electrode, which has been lifted out of the wastewater, into contact with a reaction solution containing a redox substance;
- and
- applying a voltage to the electrode in a state of being in contact with the reaction solution, and measuring a current,
- wherein, in the bringing the electrode into contact with the wastewater, the protein with which a surface of the electrode is modified and the antibody of interest are bound.
A method of detecting an antibody of interest as an infectious disease marker in wastewater, the method comprising:
- bringing an electrode, modified with a protein that specifically binds with the antibody of interest, into contact with the wastewater;
- bringing the electrode into contact with a reaction solution containing a redox substance;
- and
- applying a voltage to the electrode in a state of being in contact with the reaction solution, and measuring a current,
- wherein the redox substance is at least one kind selected from the group consisting of:
- a hexacyanidoferrate(II) ion and/or a hexacyanidoferrate(III) ion;
- an EDDHA-iron complex;
- a DTPA-iron complex;
- and salts thereof.
Dependencies are inferred from the claim text.