Device for detecting and identifying analytes in a multicomponent environment and method for manufacturing same

An integrated circuit designed for detecting and identifying analytes in a multicomponent environment, comprising

• at least one first microfluidic channel integrated into at least a part of the integrated circuit surface,
• wherein the first microfluidic channel has an inner surface and an outer surface, and
• at least part of the outer surface is made in the form of a porous hydrophobic membrane;
• at least one ion-selective transistor with a sensitive surface,
• wherein the sensitive surface is located in the first microfluidic channel under the porous hydrophobic membrane, and
• at least one pad of the transistor sensitive surface is functionalized with at least one biological receptor type for binding an analyte of interest;
• and
• a circuit for transistor signals processing, and
• wherein the sensitive surface of the transistor is located on the surface of the transistor that is part of one of the surfaces of the first microfluidic channel.

A device for detecting and identifying analytes in a multicomponent environment, comprising

• a substrate,
• an integrated circuit located on top of the substrate, and
• an encapsulating layer located on top of the integrated circuit,
• wherein the integrated circuit comprises:
• at least one first microfluidic channel integrated into at least a part of the integrated circuit surface, wherein the first microfluidic channel has an outer surface, and at least part of the outer surface is made in the form of a porous hydrophobic membrane;
• a plurality of ion-selective transistors and a plurality of ion-selective transistor sensitive surfaces arranged in matrix form, wherein the sensitive surfaces are located in the first microfluidic channel under the porous hydrophobic membrane, and at least one pad of the transistor sensitive surfaces is functionalized with at least one biological receptor type for binding an analyte of interest;
• and
• a circuit for transistor signals processing,
• wherein an internal surface of the first microfluidic channel is covered with hydrophilic materials characterized by low adsorption to analyte, and
• wherein at least one medium supply channel and at least one second microfluidic channel are located in the encapsulating layer so that the second microfluidic channel is connected to the first microfluidic channel of the integrated circuit, and the medium supply channel is located in a region proximate to the porous hydrophobic membrane of the integrated circuit.