Point-of-care testing (POCT) fully-automatic chemiluminescence device based on multi-channel parallel pretreatment technology

A point-of-care testing (POCT) fully-automatic chemiluminescence device based on a multi-channel parallel pretreatment technology, comprising

• a support;
• a reaction chamber assembly provided on the support and configured to accommodate reagent strips, wherein the reaction chamber assembly comprises a guide rail provided at a bottom of the support;
• two sides of the guide rail are respectively provided with a reaction chamber stepping motor and a base, and the reaction chamber stepping motor and the base are fixedly connected to the support;
• an output end of the reaction chamber stepping motor is provided with a reaction chamber driving pulley, and the base is provided with a reaction chamber driven pulley;
• and the reaction chamber driving pulley is connected to the reaction chamber driven pulley through a first belt;
• a reaction chamber body is provided on the guide rail;
• a bottom of the reaction chamber body is provided with a reaction chamber slider, wherein the reaction chamber slider is connected to the guide rail in a slidable manner;
• a side wall of the reaction chamber body is provided with a first synchronous belt pressing plate configured to be in contact with the first belt;
• and the belt drives the reaction chamber body to slide in a length direction of the guide rail through the first synchronous belt pressing plate;
• and
• a multi-channel parallel pretreatment assembly and a photomultiplier tube (PMT) assembly sequentially arranged on the support from front to back, wherein the multi-channel parallel pretreatment assembly and the PMT assembly are located above the reaction chamber assembly;
• the multi-channel parallel pretreatment assembly comprises a magnetic separation and transfer device configured to perform separation and transfer and a first drive device configured to drive the magnetic separation and transfer device to move;
• the magnetic separation and transfer device comprises a second mounting bracket;
• the second mounting bracket is provided with an injector chamber having a closed chamber;
• a first mounting plate is provided at a top of the injector chamber, and the first mounting plate is provided with multiple piston rods, wherein the piston rods are connected to the injector chamber in a movable and sealing manner;
• each piston rod cooperates with the injector chamber to define an independent, sealed metering cylinder configured to generate negative pressure for aspiration of a sample or reagent;
• and the second mounting bracket is further provided with a second drive device, wherein the second drive device is configured to drive the first mounting plate to linearly reciprocate in a vertical direction;
• the injector chamber is provided with loading heads, wherein each loading head has a hollow interior structure dimensioned to sequentially and interchangeably receive, by interference fit, a disposable film breaking piece, a disposable magnetic separation sleeve, and a tip head configured to receive the sample or reagent;
• and
• free ends of the piston rods are fixedly connected to magnetic rods, wherein the magnetic rods are configured to adsorb magnetic beads in a magnetic particle reagent;
• and the second drive device is configured to drive the magnetic rods on the piston rods to pass through the loading heads;
• and
• wherein the multi-channel parallel pretreatment assembly is configured to transfer, clean, and separate reagents in the reagent strips in the reaction chamber assembly to obtain cleaned and separated reagents;
• the reaction chamber assembly transfers the cleaned and separated reagents to the PMT assembly;
• and the PMT assembly is configured to detect a luminescence value of the cleaned and separated reagents;
• and
• wherein the reagent strips each are provided with a handle;
• a tip head, a disposable magnetic separation sleeve, and a disposable film breaking piece are arranged beside the handle;
• and
• the reagent strips each are provided with a first diluent hole, a second diluent hole, a magnetic bead hole, a first reagent hole, a second reagent hole, a third reagent hole, a first cleaning solution hole, a second cleaning solution hole, a third cleaning solution hole, a fourth cleaning solution hole, and a substrate reading hole side by side.