Flow cytometry immunoprofiling of peripheral blood

A method for determining a leukocyte immunoprofile type of a subject having, suspected of having, or at risk of having cancer, the method comprising:

• using at least one computer hardware processor to perform:
• obtaining flow cytometry data for white blood cells (WBC) isolated from a biological sample obtained from the subject;
• processing the flow cytometry data to determine cell composition percentages for at least 20 of the following cell types:
• Naive CD4+ Tregs, Naive CD4+ T cells, Naive CD8+ T cells, Naive B cells, Non-switched memory IgM B cells, Vδ2+ yδ T cells, Class-switched memory B cells, Central memory CD8+ T cells, CD4+ Tregs, Transitional memory CD4+ T cells, Central memory CD4+ T cells, memory CD4+ T cells, CD4+ T cells, CD39+ CD4+ Tregs, Eosinophils, Basophils, Plasmacytoid dendritic cells, Dendritic cells, PD1high CD8+ T cells, Transitional memory CD8+ T cells, Cytotoxic NK cells, Regulatory NK cells, memory CD8+ T cells, CD8+ T cells, Effector memory CD4+ T cells, NKT cells, CD8+ TEMRA, Effector memory CD8+ T cells, CD4+ TEMRA, Neutrophils, Granulocytes, Classical monocytes, Non-classical monocytes, and HLA-DRlow monocytes;
• generating a leukocyte signature for the subject using the determined cell composition percentages for the at least 20 cell types, the leukocyte signature comprising the cell composition percentages for the at least 20 cell types;
• identifying, using the leukocyte signature and from among a plurality of leukocyte immunoprofile types, a Primed leukocyte immunoprofile type for the subject;
• and
• administering an immune checkpoint inhibitor (ICI) to the subject when the subject is identified as having the Primed leukocyte immunoprofile type.