Method for preparing an injectable plasma gel from a sample of centrifuged blood

A method for preparing an injectable plasma gel from a sample of centrifuged blood, comprising:

• providing a heat resistant tube containing a sample of whole blood which has been centrifuged to form a plasma layer and a red blood cell layer, the heat resistant tube having an inner diameter;
• heating the heat resistant tube to between 167 degrees Fahrenheit and 212 degrees Fahrenheit for 5-20 minutes creating a layer of denatured plasma and a layer of denatured red blood cells;
• providing an assembly including:
• a syringe having a barrel and a plunger movably seated within the barrel, the barrel is generally cylindrical and has a hollow interior for accommodating the plunger, a proximal end of the barrel has an enlarged opening and a distal end of the barrel tapers to a tip having a lumen in fluid communication with the hollow interior, the plunger at least partially inserted into the barrel of the syringe;
• and
• a tube seal mounted on the distal end of the barrel, the tube seal having an outer diameter which closely approximates the inner diameter of the heat resistant tube, the tube seal having a lumen;
• wherein the assembly has a proximal end and a distal end, the tube seal is proximal to the distal end of the assembly;
• inserting the distal end of the assembly into the heat resistant tube such that the tube seal abuts the layer of denatured plasma;
• and
• advancing the assembly into the heat resistant tube until all of the denatured plasma layer is transferred into the hollow interior of the syringe barrel via the lumen of the tube seal and the lumen in the barrel tip;
• wherein the assembly further comprises an elongate transfer tube having a proximal end and a distal end and a lumen extending therebetween, the elongate transfer tube having on the proximal end a Luer-compatible female taper, the tube seal is mounted onto the distal end of the elongate transfer tube, the distal end of the barrel is connected to the proximal end of the transfer tube.