3D cell washer
AssigneeAgilent Technologies, Inc.
InventorsChristopher Many, Brad Larson, Byron Smith, Brian Struhammer
Life of this patent
Estimating the term…
Abstract
An autoclaving microplate washing system for cells and non-adhering three-dimensional (3D) cell cultures includes one or more pumps for controlling the dispensing of washing fluid and the evacuation of fluid from microwells to gently wash the cells. A method of controlling the autoclaving microplate washing system includes controlling the one or more pumps for dispensing and evacuation.
Claims20
A method of controlling a microplate cell washing system comprising a microplate carrier configured to support a microplate, the microplate comprising a plurality of microplate wells, a dispensing pump, a dispensing pump cassette comprising a plurality of dispense lines directly connected to the dispensing pump and terminating in dispense pipes connected to the plurality of dispense lines, the dispensing pump configured to control a first flow rate of a solution from a fluid reservoir through the plurality of dispense lines, an aspirating peristaltic pump, and an aspirating peristaltic pump cassette comprising a plurality of aspiration lines directly connected to the aspirating peristaltic pump and terminating in aspiration pipes connected to the plurality of aspiration lines, the aspirating peristaltic pump configured to control a second flow rate of a waste solution through the plurality of aspiration lines, the method comprising:
- controlling the dispensing pump to control the first flow rate of the solution from the fluid reservoir through the plurality of dispense lines for output via the dispense pipes in microplate wells corresponding to positions of the dispense pipes;
- and
- controlling the aspirating peristaltic pump to control the second flow rate of the waste solution through the plurality of aspiration lines for aspiration via the aspiration pipes in microplate wells corresponding to positions of the aspiration pipes.
A non-transitory computer-readable medium storing computer instructions that are configured to, when executed by at least one processor, cause the at least one processor to:
- control a dispensing pump, of a microplate cell washing system, to control a first flow rate of a solution from a fluid reservoir, of the microplate cell washing system, through a plurality of dispense lines in a dispensing pump cassette, of the microplate cell washing system, for output via dispense pipes, of the microplate cell washing system, in microplate wells corresponding to positions of the dispense pipes;
- and
- control an aspirating peristaltic pump, of the microplate cell washing system, to control a second flow rate of waste solution through a plurality of aspiration lines in an aspirating peristaltic pump cassette, of the microplate cell washing system, for aspiration via aspiration pipes, of the microplate cell washing system, in microplate wells corresponding to positions of the aspiration pipes.
Dependencies are inferred from the claim text.