Back to results
US 12,584,170 B2Granted
Method of nanopore sequencing of concatenated nucleic acids
AssigneeOxford Nanopore Technologies PLC
InventorsAndrew John Heron, Daniel Ryan Garalde, James White
Life of this patent
Estimating the term…
Abstract
The invention relates to a new method of characterising two or more target polynucleotides using a pore. The method involves sequentially attaching to a first polynucleotide one or more subsequent polynucleotides to form a concatenated polynucleotide.
Claims18
A population of polynucleotide adaptors comprising a first polynucleotide adaptor and a second polynucleotide adaptor,
- wherein the first polynucleotide adaptor and the second polynucleotide adaptor each comprise a first polynucleotide strand and a second polynucleotide strand, each strand having a 5′ end and a 3′ end, wherein:
- (a) a portion extending to the 3′ end of the first polynucleotide strand is complementary to a portion extending to the 5′ end of the second polynucleotide strand, and the complementary portions form a duplex;
- (b) a single stranded portion extending to the 5′ end of the first polynucleotide strand and a single stranded portion extending to the 3′ end of the second polynucleotide strand are not complementary and do not hybridise to one another;
- (c) the complementary portion extending to the 5′ end of the second polynucleotide strand of the first polynucleotide adaptor comprises a sequence that is capable, when the duplex is unwound, of hybridising to a sequence comprised in the single stranded portion extending to the 5′ end of the first polynucleotide strand of the second polynucleotide adaptor;
- and
- (d) the 5′ end of the first polynucleotide strand comprises a click reactive group and the 3′ end of the second polynucleotide strand comprises a complementary click reactive group.
3 more elements
A population of polynucleotide adaptors comprising a first polynucleotide adaptor and a second polynucleotide adaptor,
- wherein the first polynucleotide adaptor and the second polynucleotide adaptor each comprise a first polynucleotide strand and a second polynucleotide strand, each strand having a 5′ end and a 3′ end, wherein:
- (a) a portion extending to the 3′ end of the first polynucleotide strand is complementary to a portion extending to the 5′ end of the second polynucleotide strand, and the complementary portions form a duplex;
- (b) a single stranded portion extending to the 5′ end of the first polynucleotide strand and a single stranded portion extending to the 3′ end of the second polynucleotide strand are not complementary and do not hybridise to one another;
- (c) the complementary portion extending to the 5′ end of the second polynucleotide strand of the first polynucleotide adaptor comprises a sequence that is capable, when the duplex is unwound, of hybridising to a sequence comprised in the single stranded portion extending to the 5′ end of the first polynucleotide strand of the second polynucleotide adaptor;
- and
- (d) the 5′ end of the first polynucleotide strand comprises a phosphatase.
3 more elements
Dependencies are inferred from the claim text.