Stabilization of nucleic acids in urine
AssigneeStreck, Inc.
InventorsJianbing Qin, Bradford A. Hunsley
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Abstract
A composition and method for preserving a urine sample and a preservative delivery vessel are disclosed wherein treatment of the urine sample aids in preserving circulating cell-free nucleic acids in urine over a wide range of dilution ratios within temperature fluctuations that can occur during urine sample handling, storage and transportation. The urine sample preservation composition and method and preservative delivery vessel provide a method for obtaining high quality stabilized urinary cell-free nucleic acids for clinical diagnostics development and application.
Claims26
A composition comprising:
- (a) a urine sample;
- (b) a nuclease inhibitor present in a concentration of at least 0.5% of the composition;
- (c) a preservative present in a concentration of from about 0.2% to about 4% of the composition;
- (d) a formaldehyde quencher present in a concentration of at least 0.01%, wherein a ratio of nuclease inhibitor to preservative is about 9:10 or about 9:20.
A specimen container comprising a urine preservative composition for maintaining structural integrity of nucleated blood cells and cell-free nucleic acids in a urine sample comprising:
- (a) a nuclease inhibitor present in an amount of from about 100 to about 300 g/L of the composition;
- (b) a preservative;
- (c) a formaldehyde quencher, wherein the ratio of nuclease inhibitor to preservative is about 9:10 or about 9:20.
Dependencies are inferred from the claim text.