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US 12,584,156 B2Granted
Method for producing protein
AssigneeAjinomoto Co., Inc.
InventorsYasumichi Koseki, Takahiro Katayama, Yusuke Ooya, Fumi Ogata, Kiyoshi Hirakawa, Takuya Higuchi
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Abstract
A method for producing an objective protein using animal cells as an expression host is provided. The objective protein is produced by culturing animal cells having an objective protein-producing ability under conditions where the phosphoric acid concentration and/or the potassium concentration in the culture medium is increased.
Claims22
A method for producing an objective substance, the method comprising:
- culturing animal cells having an objective substance-producing ability in a culture medium;
- and
- collecting the objective substance,
- wherein the culturing is carried out under the following condition:
- (A) a phosphoric acid concentration in the culture medium is increased to be 5 mM or more;
- and/or
- (B) a phosphoric acid concentration in the culture medium is increased to be 5 mM or more and a potassium concentration in the culture medium is increased to be 1 mM or more.
4 more elements
A method for culturing animal cells, the method comprising:
- culturing the animal cells in a culture medium,
- wherein the culturing is carried out under the following condition:
- (A) a phosphoric acid concentration in the culture medium is increased to be 5 mM or more;
- or
- (B) a phosphoric acid concentration in the culture medium is increased to be 5 mM or more and a potassium concentration in the culture medium is increased to be 1 mM or more.
2 more elements
Dependencies are inferred from the claim text.