Modified microorganisms to increase yield of xylose-derived products
AssigneeNational Technology & Engineering Solutions of Sandia, LLC
InventorsPaul Alexander Adamczyk, John Michael Gladden, Samuel Coradetti
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Abstract
Methods and engineered hosts are disclosed that convert a lignocellulosic xylose-containing biomass source into xylonic acid and/or xylonate, which can be further processed into other useful derivatives. In particular, an exemplary engineered Bacidiomycetes, e.g., R. toruloides, host produces/expresses one or more fungal enzymes that convert xylose into xylonic acid/xylonate. Methods of using such hosts to consume pretreated lignocellulosic biomass in combination with certain native promoters and heterologous genes are also described herein.
Claims20
An engineered organism comprising an engineered host derived from a Basidiomycete organism having a gene with at least 95% sequence identity to SEQ ID NO:
- 1 in its genome, the engineered host having the gene with 95% or greater sequence identity to SEQ ID NO:
- 1 deleted from its genome;
- wherein XylB and XylC are expressed in the engineered host;
- and
- the engineered organism is capable of producing xylonic acid, xylonate, or a combination thereof.
A fermentation broth composition, comprising an energy source comprising xylose and an engineered host, the engineered host being derived from a Basidiomycete organism having a gene with at least 95% sequence identity to SEQ ID A NO:
- 1 in its genome, and the engineered host having the gene with at least 95% sequence identity to SEQ ID NO:
- 1 deleted from its genome;
- wherein XylB and XylC are expressed in the engineered host;
- and
- the fermentation is capable of producing xylonate, or a combination thereof.
A method of making a xylose-derived product via a fermentation broth, comprising:
- expressing XylB and XylC in the fermentation broth via introducing promoter sequences for expressing XylB and XylC in an engineered host:
- combining an energy source comprising xylose and the engineered host, the engineered host being derived from a Basidiomycete organism having a gene with at least A 95% sequence identity to SEQ ID NO:
- 1 in its genome, and the engineered host A having the gene with at least 95% sequence identity to SEQ ID NO:
- 1 deleted from its genome;
- and
- the method produces xylonic acid, xylonate, or a combination thereof.
Dependencies are inferred from the claim text.