Endogenous lipase for metal reduction in distillers corn oil
AssigneePOET Research, Inc.
InventorsAndrew J. Manning, Alex T. McCurdy, Steven T. Bly
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Abstract
Provided herein are compositions, methods, systems associated with propagation and fermentation, and co-products of biochemical production processes, for example, a DCO co-product resulting from converting oil containing grains into bio chemicals via fermentation in the presence of endogenous esterase. The DCO resulting from the processes exhibits lower metal ion content relative to a DCO obtained in the absence of endogenous fermentation with an esterase such as a lipase. The DCO is useful as a feedstock for the production of renewable diesel.
Claims18
A method of producing distillers corn oil (DCO), the method comprising:
- (a) inoculating a feedstock:
- (i) with a combination of a first microorganism which is an ethanologen and a second microorganism genetically engineered to produce esterase;
- or
- (ii) with an ethanologen, wherein the ethanologen is a yeast genetically modified to produce esterase;
- and
- (b) fermenting the feedstock in the presence of produced esterase to produce ethanol and DCO,
- wherein the esterase is present in an amount sufficient to reduce (i) free fatty acid (FFA) content and (ii) metal ion content or phosphorus content, in DCO,
- wherein the DCO comprises decreased FFA content relative to the FFA content of a DCO produced in the absence of an esterase-producing microorganism or genetically modified yeast;
- and
- wherein the metal ion content or phosphorus content is reduced by at least about 10% relative to fermentation in the absence of an esterase-producing microorganism or genetically modified yeast.
Dependencies are inferred from the claim text.