US 12,584,143 B2 · Combination of TIS sequence and signal peptide sequence for expressing a recombinant protein

Abstract

The present invention relates to DNA constructs comprising a novel TIS sequence. The TIS sequence transcribes into an RNA motif that functions as the protein translation initiation site in an mRNA transcript. The DNA construct may further comprise a nucleic acid sequence encoding signal peptide. Additionally, the DNA constructs may also comprise a nucleic acid sequence encoding a recombinant protein or one or more polypeptide chains thereof. The invention further relates to an expression vector, expression cassette and host cell which comprise said DNA constructs. Furthermore, the present invention relates to a recombinant protein expressed by the host cell as well as a method for expressing the recombinant protein.

Claims25

Dependent claims

A DNA construct for expressing a recombinant protein in mammalian cells, wherein the DNA construct comprises:

a nucleic acid sequence of SEQ ID No. 1, wherein the nucleic acid sequence of SEQ ID No. 1 is a TIS sequence;
and
a nucleic acid sequence which encodes a signal peptide, wherein the nucleic acid sequence which encodes the signal peptide comprises a nucleic acid sequence of SEQ ID No 2;
and wherein the nucleic acid sequence of SEQ ID No. 1 comprises:
the ATG start codon in the nucleic acid sequence which encodes the first amino acid residue of the signal peptide;
and
the first two nucleotides downstream of the ATG start codon in the nucleic acid sequence which encodes the second amino acid residue of the signal peptide.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/911,772Filed 17 Mar 2021

Priority

SE 2030080-217 Mar 2020

Published

US 2023/0175008 A18 Jun 2023

Granted

24 Mar 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 15/67
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: general methods for enhancing…
C12N 15/85
Vectors or expression systems specially adapted for eukaryotic hosts: for animal cells
C07K 16/2818
Against the immunoglobulin superfamily: against CD28 or CD152
C12N 2830/34
Vector systems having a special element relevant for transcription: being a transcription initiation element

Combination of TIS sequence and signal peptide sequence for expressing a recombinant protein

Life of this patent

Combination of TIS sequence and signal peptide sequence for expressing a recombinant protein

Life of this patent

Abstract

Claims25