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AssigneeNorthwest A&F University
InventorsJun Guo, Fuxin He, Zhensheng Kang, Huilin Sun, Xingxuan Bai, Ce Wang, Yanfeng Wang
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A method for improving wheat resistance to Fusarium head blight (FHB) by genome editing is provided, and further provided is use of TaCIPK14 protein or related biomaterial thereto in the regulation of plant resistance to FHB; the TaCIPK14 protein is TaCIPK14-4A protein (SEQ ID NO: 4), TaCIPK14-4B protein (SEQ ID NO: 5), and/or TaCIPK14-4D protein (SEQ ID NO: 6). TaCIPK14 knockout mutant plants are successfully obtained by simultaneously editing three alleles of TaCIPK14 in wheat using CRISPR-Cas9 gene editing technology. The TaCIPK14 knockout mutant shows resistance to inoculation with FHB pathogens, and the results of evaluation of major agronomic traits confirm that the TaCIPK14 knockout mutant reserves the major agronomic traits.
A method for increasing wheat resistance to Fusarium Head Blight (FHB), said method comprising knocking out a TaCIPK14 genes to decrease expression and/or activity of TaCIPK14 protein in a wheat plant;
A method for breeding a wheat variety with improved resistance to Fusarium Head Blight (FHB), said method comprising knocking out a TaCIPK14 genes to reduce the expression and/or activity of TaCIPK14 protein in a wheat plant;
A method for cultivating transgenic wheat with improved resistance to FHB, comprising steps of inhibiting expression of a nucleic acid molecule capable of expressing TaCIPK14 protein in a wheat plant, and obtaining the transgenic wheat with improved resistance to FHB;
Dependencies are inferred from the claim text.