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US 12,584,125 B2Granted
Methods and use of chimeric proteins
AssigneeThe Regents of the University of California
InventorsEugene Yeo, Kristopher W. Brannan, Ryan Marina, Isaac A. Chaim, Daniel A. Lorenz
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Abstract
Provided are methods for using chimeric proteins to produce RNA modifications that can be detected by sequencing methods, including methods detecting relative translation rates of various mRNAs. Also provided herein are compositions comprising chimeric proteins, wherein the chimeric proteins comprise a RNA editing protein and a ribosomal protein.
Claims12
A method for determining a relative translation rate of a target mRNA in a cell, as compared to a non-target mRNA, the method comprising:
- (a) introducing into the cell a chimeric protein comprising (i) a ribosomal protein consisting of RPS2 or RPS3 and (ii) an RNA editing protein;
- (b) determining a plurality of nucleotide substitutions introduced into the target mRNA by the RNA editing protein;
- and
- (c) comparing the determined plurality of nucleotide substitutions introduced into the target mRNA to a plurality of nucleotide substitutions introduced into the non-target mRNA in the cell, thereby determining the relative translation rate of the target mRNA in the cell, as compared to the non-target mRNA.
1 more element
Dependencies are inferred from the claim text.