Magnetic bead based nucleic acid extraction system

A magnetic bead based nucleic acid extraction system, comprising:

• a plurality of magnetic beads, and
• an alcohol-free buffer system, comprising:
• a lysis binding buffer comprising 0.5 to 4 M GuSCN, 5 to 20% (v/v) PEG8000, 10 to 1000 mM sodium citrate, and a non-ionic detergent selected from the group consisting of 0.5 to 4% (v/v) nonylphenyl polyethylene glycol, 0.5 to 4% (v/v) secondary alcohol ethoxylate, 0.5 to 4% (v/v) ethylene oxide-propylene oxide copolymer mono(2-ethylhexyl) ether and mixtures thereof;
• a first wash buffer comprising 0.5 to 3 M GuSCN, 5 to 20% (v/v) PEG8000, 0.5 to 2 M sodium chloride, 5 to 25 mM EDTA, and a non-ionic detergent selected from the group consisting of 0.25 to 4% (v/v) ethylene oxide-propylene oxide copolymer mono(2-ethylhexyl) ether, 0.1 to 4% (v/v) 2-[4-(2,4,4-trimethylpentan-2-ylphenoxy]ethanol, 0.25 to 4% (v/v) secondary alcohol ethoxylate and mixtures thereof;
• a second wash buffer comprising 1 to 25 mM hexammine cobalt (III) chloride, 5 to 20% (v/v) PEG8000, 5 to 1000 mM sodium chloride, and a non-ionic detergent selected from the group consisting of 0.25 to 2% (v/v) ethylene oxide propylene oxide copolymer mono(2-ethylhexyl) ether, 0.1 to 2% (v/v) 2-[4-(2,4,4-trimethylpentan-2-yl phenoxy]ethanol, 0.25 to 2% (v/v) secondary alcohol ethoxylate and mixtures thereof;
• and
• an elution buffer.