Use of gene encoding gibberellin 3beta-hydroxylase of Glycine max, GmGA3ox1
AssigneeNanjing Agricultural University
InventorsDeyue Yu, Fang Huang, Dezhou Hu, Hui Wang, Zhongyi Yang, Shaoqi Lu, Xiao Li
Life of this patent
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Abstract
Provided is a use of gene encoding gibberellin 3B-hydroxylase of G. max, GmGA3ox1. The use of gibberellin 3β-hydroxylase gene of G. max, GmGA30x1, set forth in SEQ ID NO:1, is in genetic engineering of seed weight of Arabidopsis thaliana and Glycine max. In A. thaliana, overexpression of GmGA3ox1 can complement the low seed weight phenotype of an atga3ox1 mutant. In G. max, overexpression of the excellent haplotype of the gene can significantly improve the seed weight of G. max.
Claims2
A method for genetic engineering of seed weight or length of Glycine max, comprising using a sequence of Glycine max;
- wherein the method for genetic engineering comprises:
- using DNA of leaves of Jurong Flat Green Bean as a template, amplifying the DNA using primers set forth in SEQ ID NO:
- 19 and SEQ ID NO:
- 20 to obtain a sequence of Glycine max with a length of 4187 bp, ligating the sequence of Glycine max into a pCAMBIA3301 vector without a CaMV 35S promoter to obtain a pCAMBIA3301-GmGA3ox1 plant overexpression vector, wherein a representative sample has been deposited under CGMCC deposit number 34555, transferring the pCAMBIA3301-GmGA3ox1 plant overexpression vector into Agrobacterium tumefaciens strain EHA105, and using the resulting EHA105 transformant to transform Glycine max cultivar Williams 82.
A method for improving the yield of Glycine max, comprising the following steps:
- overexpressing a protein of Glycine max or a gene encoding the protein of Glycine max, in Glycine max;
- wherein the overexpressing step comprises:
- using DNA of leaves of Jurong Flat Green Bean as a template, amplifying the DNA using primers set forth in SEQ ID NO:
- 19 and SEQ ID NO:
- 20 to obtain a sequence of Glycine max with a length of 4187 bp, ligating the sequence of Glycine max into a pCAMBIA3301 vector without a CaMV 35S promoter to obtain a pCAMBIA3301-GmGA3ox1 plant overexpression vector, wherein a representative sample has been deposited under CGMCC deposit number 34555, transferring the pCAMBIA3301-GmGA3ox1 plant overexpression vector into Agrobacterium tumefaciens strain EHA105, and using the resulting EHA105 transformant to transform Glycine max cultivar Williams 82.
Dependencies are inferred from the claim text.