Method of cell culture
AssigneePfizer Inc.
InventorsGregory Walter Hiller, Bhanu Chandra Mulukutla
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Abstract
A method of cell culture comprising providing cells in a cell culture medium to start a cell culture process, and, maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenylpyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3 mM and/or (ii) maintaining at least one amino acid selected from phenylalanine, tyrosine, tryptophan, methionine, leucine, serine, threonine and glycine below a concentration C2 in the cell culture medium, wherein C2 is 2 mM.
Claims9
A method of cell culture comprising
- (i) providing Chinese hamster ovary (CHO) cells in a cell culture medium and starting a fed-batch cell culture process with the cells in the cell culture medium,
- wherein the cell culture medium comprises histidine at a concentration above 2 mM,
- wherein the concentration of serine is at a concentration below 1 mM at the start of the culture process, and
- wherein the fed-batch cell culture process comprises adding more serine to the cell culture medium at one or more times after starting the cell culture process and,
- (ii) maintaining serine below a concentration of 1 mM in the cell culture medium and
- wherein step (ii) comprises a step of measuring the concentration of serine in the cell culture medium prior to adding more serine to the cell culture medium and based on the measured concentration of serine in the cell culture medium, adding an amount of serine to the cell culture medium such that after adding the amount of serine, the concentration of serine is below 1 mM in the cell culture medium.
Dependencies are inferred from the claim text.