Functional feline pancreatic cells from adipose tissue

A cell culture derived from a population of adult feline adipose multipotent stromal cells (ASCs), comprising:

• a mixed population of pancreatic hormone-expressing islet-like cells that comprises pancreatic precursor cells, insulin-producing cells, pancreatic cells or a combination thereof;
• and
• a culture medium comprising an effective amount of insulin-transferrin-selenium, taurine, glucagon-like peptide 1 (GLP-1), nicotinamide, pentagastrin, and betacellulin, mammalian hepatocyte growth factor (HGF), basic fibroblast growth factor (bFGF), and at least one component selected from the group consisting of glucose, bovine serum albumin, non-essential amino acids, N-2 supplement, a neuron-induction culture supplement,
• wherein the culture medium further comprises an effective amount of laminin to generate the mixed population of pancreatic hormone-expressing islet-like cells that can express insulin in response to a glucose stimulus in a dose dependent manner and can express glucagon and somatostatin.