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AssigneeEuropean Molecular Biology Laboratory
InventorsMelanie Tramontano, Kiran Raosaheb Patil, Martina Klünemann, Mihaela Pruteanu, Lisa Maier, Michael Kuhn, Sergej Andrejev, Yongkyu Kim, Peer Bork, Athanasios Typas, Georg Zeller
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The present invention relates to an in-vitro model of the human gut microbiome, the model comprising a culture of the gut microbiome, wherein the model has a cumulative enzymatic coverage of more than 85% of the gut microbiome of a healthy human. The model facilitates metabolic modeling and enables a better understanding of the structure and function of the human gut microbiome as well as of modifications of xenobiotics by intrinsic gut microbiota, such as biotransformation and bioaccumulation. It can further be used to study the effects of variations in nutritional conditions. Importantly, the invention can also be used for diagnosing a disease, such as a gastrointestinal disorder, a proliferative disease, a metabolic disorder, a cardiovascular disease, an immunological disease, and an infectious disease.
An in-vitro model of the human gut microbiome, comprising a panel consisting of bacterial species selected from Bacteroides, Eubacterium, Alistipes, Ruminococcus, Roseburia, Parabacteroides, Prevotella, Bifidobacterium, Coprococcus, Dorea, Blautia, Odoribacter, Clostridium, Streptococcus, Collinsella, and Bilophila, and a medium kit for culturing bacteria of the human gut microbiome, wherein the medium kit comprises at least one of defined gut microbiota medium (dGMM), lactic acid bacteria (LAB) medium, dGMM and LAB medium, dGMM and LAB medium comprising a reduced amount of minerals and vitamins, dGMM and LAB medium excluding short chain fatty acids (SCFA), dGMM and LAB medium comprising monosaccharides as only carbohydrate source, dGMM and LAB medium comprising Mucin, dGMM and LAB medium comprising Mucin as only carbohydrate source, dGMM and LAB medium comprising only 10% amino acids, and dGMM and LAB medium excluding aromatic amino acids, and wherein said panel has a cumulative enzymatic coverage of more than 85% of the gut microbiome of a healthy human.
Dependencies are inferred from the claim text.