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AssigneeCorbion Biotech, Inc.
InventorsJohn Piechocki, Cornelis Johannes Govardus Van Strien, Linda Wilhelmina Antonetta Claassen, Peter Johannes Marie Baets, Jorge Galazzo
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A process for manufacturing a lysed cell in oil suspension and methods of applying thereof, the lysed cell in oil suspension including a solids content of at least 10 wt. % and a particle size Dv90 of 100 to 300 microns, including steps a) adding a first portion of microbial cell matter to an oil, b) subjecting the mixture of microbial cell matter and oil to one or more shear steps to effect lysis of at least part of the cells and produce a suspension including lysed cells in oil, c) adding a further portion of microbial cell matter to the suspension including lysed cells in oil, d) subjecting the mixture of microbial cell matter and the suspension including lysed cells in oil to one or more shear steps to effect lysis of at least part of the cells and produce a further suspension including lysed cells in oil.
A process for manufacturing a lysed cell in oil suspension with a solids content of at least 10 wt. %, a water content of at most 10 wt. %, and a bimodal particle size distribution with a first peak having a Dv50 value of 3 to 6 microns, and a second peak having a Dv50 value of 80 to 200 microns, the process comprising:
A lysed cell in oil suspension having a solids content of at least 10 wt. %, a water content of at most 10 wt. %, and a bimodal particle size distribution with a first peak having a Dv50 value of 3 to 6 microns, and a second peak having a Dv50 value of 80 to 200 microns, wherein the suspension comprises lysed cell matter and oil.
Dependencies are inferred from the claim text.