Highly durable permeable fluoropolymer cell culture bag

A cell culture bag comprising:

• a tube extending from a first end to a second end and defining a cell culture compartment,
• wherein the tube is formed of a first composite film comprising a first fluoropolymer and a second fluoropolymer,
• wherein the first fluoropolymer has a first thickness,
• wherein the second fluoropolymer at least partially impregnates the first thickness of the first fluoropolymer,
• wherein the first composite film has a second thickness of 0.01 mm to 0.059 mm,
• wherein the first composite film has an O2 flux of 2,000 cm3/m2/atm/day to 20,000 cm3/m2/atm/day,
• wherein the first composite film has a tensile strength of 10,000 psi to 92,000 psi, and
• wherein the first composite film has a total transmittance of at least 70%, as measured according to ASTM D1003-13;
• a first joint at a first end;
• a second composite film configured to be folded over the first joint to form a first lap seam over the first joint;
• and
• a second lap seam extending longitudinally from the first end to the second end,
• wherein the second lap seam is formed by overlapping edges of the first composite film.

A cell culture assembly, comprising:

• a plurality of cell culture bags connected in series, each of the plurality of cell culture bags comprising:
• a body formed of a composite film comprising a first fluoropolymer and a second fluoropolymer,
• wherein the body defines a cell culture compartment configured to hold a cell culture,
• wherein the first fluoropolymer has a first thickness,
• wherein the second fluoropolymer at least partially impregnates the first thickness of the first fluoropolymer,
• wherein the composite film has a second thickness from 0.01 mm to 0.059 mm,
• wherein the composite film has a tensile strength in one direction from 10,000 psi to 92,000 psi,
• wherein the composite film has an O2 flux from 2,000 cm3/m2/atm/day to 20,000 cm3/m2/atm/day, and
• wherein a total transmittance of the composite film is from 70% to 100%, as measured according to ASTM D1003-13.