Antibody specific to Staphylococcus aureus, therapeutic method and detection method using same
OwnerWilliam R. Churchinventor
Life of this patent
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Abstract
We provide new monoclonal antibody inhibitors of coagulases, staphylocoagulase (“SC”) and von Willebrand factor-binding protein (“vWbp”) for treatment of S. aureus. The monoclonal antibodies are useful in targeting the SC N-terminus of SC and vWbp (respectively) and inhibiting prothrombin activation. The monoclonal antibodies are able to bind to and interfere with, modulate, and/or inhibit the binding interactions between the coagulase protein and its ligan protein prothrombin the activation of prothrombin.
Claims6
An isolated monoclonal antibody or fragment thereof which binds an epitope of von Willebrand factor-binding protein (“vWbp”) that is within SEQ ID. No:
- 4 and which comprises the following CDRs:
- CDRH1:
- GYSFTSYWIH
- (residues 26-35 of SEQ ID NO.:
- 29);
- CDRH2:
- AISPGNSDTNYNQNFKG
- (residues 50-66 of SEQ ID NO.:
- 29);
- CDRH3:
- ATGSHY
- (residues 97-102 of SEQ ID NO.:
- 29);
- CDRL1:
- QSLLDSDGTTY
- (residues 27-37 of SEQ ID NO.:
- 31);
- CDRL2:
- LVS
- (residues 55-57 of SEQ ID NO.:
- 31);
- and
- CDRL3:
- WQGTHFPRT
- (residues 94-102 of SEQ ID NO.:
- 31).
An isolated monoclonal antibody or fragment thereof, which binds an epitope of von Willebrand factor-binding protein (“vWbp”) that is within SEQ ID. No:
- 4 and which comprises the following CDRs:
- CDRH1:
- (residues 26-35 of SEQ ID NO.:
- 29)
- GYSFTSYWIH;
- CDRH2:
- (residues 50-66 of SEQ ID NO.:
- 29)
- AISPGNSDTNYNQNFKG;
- CDRH3:
- (residues 97-102 of SEQ ID NO.:
- 29)
- ATGSHY;
- CDRL1:
- (residues 27-37 of SEQ ID NO.:
- 31)
- QSLLDSDGTTY;
- CDRL2:
- (residues 55-57 of SEQ ID NO.:
- 31)
- LVS;
- and
- CDRL3:
- (residues 94-102 of SEQ ID NO.:
- 31)
- WQGTHFPRT;
- and
- wherein the isolated monoclonal antibody or fragment thereof is humanized.
A method of detecting the presence of S. aureus in a sample, said method comprising contacting the sample with an isolated monoclonal antibody or antigen-binding fragment thereof which binds an epitope of at vWbp peptide that is within SEQ ID. NO.:
- 4 and which comprises the following CDRs:
- CDRH1:
- GYSFTSYWIH
- (residues 26-35 of SEQ ID NO.:
- 29);
- CDRH2:
- AISPGNSDTNYNQNFKG
- (residues 50-66 of SEQ ID NO.:
- 29);
- CDRH3:
- ATGSHY
- (residues 97-102 of SEQ ID NO.:
- 29);
- CDRL1:
- QSLLDSDGTTY
- (residues 27-37 of SEQ ID NO.:
- 31);
- CDRL2:
- LVS
- (residues 55-57 of SEQ ID NO.:
- 31);
- and
- CDRL3:
- WQGTHFPRT
- (residues 94-102 of SEQ ID NO.:
- 31).
Dependencies are inferred from the claim text.