DNA-binding domain transactivators and uses thereof
AssigneeUniversity of Massachusetts
InventorsMiguel Sena Esteves, Scot A. Wolfe
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Abstract
In some aspects, the disclosure relates to recombinant adeno-associated viruses (rAAVs) comprising a nucleic acid encoding a fusion protein comprising a DNA-binding domain and a transcriptional regulator domain and methods of using the same. In some embodiments, expression of the fusion protein results in modified expression of a target gene in a cell.
Claims15
An isolated nucleic acid comprising a transgene configured to express at least one DNA binding domain fused to at least one transcriptional regulator domain, wherein the DNA binding domain binds to a target gene or a regulatory region of a target gene, wherein the target gene encodes a voltage-gated sodium channel, and wherein the at least one transcriptional regulator domain is encoded by the nucleic acid sequence set forth in SEQ ID NO:
- 47 and/or the at least one transcriptional regulator domain is encoded by the amino acid sequence set forth in SEQ ID NO:
- 48.
Dependencies are inferred from the claim text.