Separation of oligosaccharides
AssigneeGlycom A/S
InventorsNikolay Khanzhin, Markus Jondelius Hederos, Pierre Chassagne
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Abstract
The invention relates to a method for the separation of two hydrophilic neutral oligosaccharides from each other with a chromatography on a bromine functionalized polystyrene cross-linked with divinylbenzene (BPS-DVB) stationary medium.
Claims11
A method for separating a first human milk oligosaccharide (HMO) from a second HMO comprising subjecting an aqueous solution comprising the first and the second HMO to reverse phase chromatography on a bromine functionalized polystyrene cross-linked with divinylbenzene (BPS-DVB) stationary medium, wherein the second HMO is a tetrasaccharide and the first HMO is a hexa- or heptasaccharide, and wherein the first HMO and the second HMO comprise a GlcNAc-moiety.
Dependencies are inferred from the claim text.