Actuation systems and methods for use with flow cells

A system, comprising:

• a valve drive assembly;
• a reagent cartridge comprising:
• a common fluidic line;
• and
• a plurality of reagent fluidic lines, each of the plurality of reagent fluidic lines being adapted to be coupled to a corresponding reagent reservoir;
• and
• a manifold assembly comprising a plurality of membrane valves and a plurality of actuators disposed within the manifold assembly, the manifold assembly selectively fluidically coupling the common fluidic line and a corresponding one of the plurality of reagent fluidic lines responsive to the valve drive assembly actuating a corresponding one of the plurality of actuators, each of the plurality of membrane valves is formed between the common fluidic line and a corresponding reagent fluidic line, wherein each of the actuators comprises a pivot having a distal end that is adapted to move a membrane portion of a membrane of the manifold assembly away from a valve seat of the manifold assembly,
• wherein the valve drive assembly is adapted to interface with the actuators and the plurality of membrane valves to selectively control a flow of reagent between each of the plurality of reagent fluidic lines and the common fluidic line.

An apparatus, comprising:

• a common fluidic line;
• a plurality of reagent fluidic lines, each of the plurality of reagent fluidic lines being adapted to be coupled to a corresponding reagent reservoir;
• and
• a manifold assembly comprising a plurality of membrane valves and a plurality of actuators disposed within the manifold assembly, the manifold assembly selectively fluidically coupling the common fluidic line, a corresponding one of the plurality of reagent fluidic lines responsive to actuation of a corresponding one of the plurality of actuators, each of the plurality of membrane valves is formed between the common fluidic line and a corresponding one of the plurality of reagent fluidic lines, wherein at least one of the plurality of actuators is a cantilever having a distal end that is adapted to move one of opposing membranes of the manifold assembly away from a corresponding valve seat of one of the plurality of membrane valves.