Method for producing transplantable oral mucosa tissue
AssigneeMukocell GmbH
InventorGouya Ram-Liebig
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Abstract
The present invention relates to a method for producing transplantable oral mucosa tissue by selecting and choosing from oral mucosa biopsy, and to the use thereof as pharmaceutical composition, medicament or transplant or graft material, in particular for urethral reconstruction or cornea implantation. In particular, the invention relates to markers for identifying suitable transplantable oral mucosa tissue for the production of transplantable oral mucosa tissue, in particular for producing an autologous oral mucosa graft or tissue.
Claims16
A method for selecting oral mucosa tissue for production of transplantable oral mucosa tissue, wherein cells are cultured on a surface of a support, wherein at least one
- potency marker is selected from at least one vascular endothelial growth factor (VEGF) having a threshold value of more than or equal to 10 μg/ml in supernatant after 24 h of seeding on the surface
- and at least one
- purity marker is selected from at least one acidic and basic (Type I and II) subfamilies of cytokeratins, wherein the amount of epithelial cells is more than or equal to 70%, optionally 80%,
- and/or at least one
- impurity marker is selected from at least one Cluster Differentiation 90, wherein the amount of non-epithelial cells is less than or equal to 30%, optionally 20%, wherein the markers are determined in vitro.
Dependencies are inferred from the claim text.