Composition and methods for sanitization
AssigneeGenzyme Corporation
InventorsJoseph P. Kutzko, Ryan F. Wollensak
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Abstract
The present technology relates to a novel sanitization method for chromatography media and supporting equipment comprising treatment with a sanitization/sterilization solution comprising acetic acid and hexylene glycol.
Claims21
A method for sanitizing or sterilizing chromatography media and/or supporting equipment, comprising contacting the chromatography media and/or supporting equipment with a sanitization or sterilization solution comprising acetic acid and hexylene glycol, wherein
- the concentration of acetic acid is from about 40 mM to about 200 mM,
- the concentration of hexylene glycol is from about 8% by volume to about 80% by volume, and
- the pH of the sanitization or sterilization solution is ≤3.5.
A method for eluting a target analyte bound to a chromatography media, comprising contacting the chromatography media with an elution buffer comprising acetic acid and hexylene glycol, wherein
- the concentration of acetic acid is from about 40 mM to about 200 mM,
- the concentration of hexylene glycol is from about 8% by volume to about 80% by volume, and
- the pH of the elution buffer is ≤3.5.
A method of increasing the lifetime of a chromatography media, comprising sanitizing or sterilizing the chromatography media with a solution comprising about 65 mM acetic acid and about 20% by volume hexylene glycol, wherein the method allows the lifetime of the chromatography media to be increased by at least about 10% as compared to sanitizing or sterilizing the chromatography media with at least one of:
- (i) gamma irradiation or (ii) a buffer comprising sodium hydroxide.
Dependencies are inferred from the claim text.